MicroRNAs play an important role in regulating protein translation of coding genes. They can interact with long non-coding RNAs that regulate alternative splicing events, which impact processes such as apoptosis, proliferation and differentiation, all of which have shown to be deregulated and thereby causative elements in diseases such as cancer.

The Affymetrix® miRNA 4.1 Array is optimized for 203 species with more than 30.000 total mature miRNA probe sets. It is designed to interrogate all mature miRNA sequences in miRBase Release 20.


Performance specifications

miRNA 4.1

Reproducibility (inter– and intra–lot)

≥0.95

Transcripts detected at 1.0 amol

≥85%

Dynamic range

4 logs

Total RNA input

130–500 ng

Array contents

miRNA 4.1

miRBase version

20

Organisms

203

Total mature miRNA probe sets

30,424

Probes/probe set for mature miRNA

9

Human mature miRNA

2,578

Mouse mature miRNA

1,255

Rat mature miRNA

728

Human snoRNA and scaRNA

1,996

Human pre-miRNA

2,025

Mouse pre-miRNA

1,255

Rat pre-miRNA

490