Transcriptomics

Transcriptome Sequencing

RNA sequencing (RNA-seq) captures a broad range of gene expression changes and enables the detection of novel transcripts in both coding and non-coding RNA species. More specifically, sequencing the transcriptome allows for the discovery, quantification and structural profiling of gene transcripts, including mRNAs, non-coding RNAs, and small RNAs. RNA-seq makes use of high-throughput sequencing technology to sequence cDNA. Additionally, "stranded" information identifies from which of the two DNA strands a given RNA transcript was derived. It provides increased confidence in transcript annotation and allows for the differentiation between sense and antisense transcription. Thus, it is a powerful method for identifying and quantifying the entire population of RNAs in your sample.

Our transcriptome profiling service provides a comprehensive and most complete picture of the RNA content in your samples. We help you to set up and conduct high-quality, well-controlled RNA-seq experiments based on the latest deep-sequencing technologies.

Our transcriptome profiling applications include:

  • Profiling and comparison of genome-wide expression in different biological samples
  • Detection of novel transcripts
  • Detection and quantification of alternative splicing events
  • Allele specific expression analysis
  • Detection of mutation events, such as single nucleotide variations (SNVs), gene fusions, or other specific coding mutations

We use special kits for the preparation of high quality input RNA for sequencing and microarray applications. These kits allow preparation of RNA from low quality, FFPE, or ultra-low input samples, and removal of globin and rRNA.

TruSeq Stranded mRNA-Seq

TruSeq Stranded Total RNA-Seq

Globin-Reduction

TruSeq Access

Input

50 ng RNA

50 ng RNA

100 ng RNA

50 ng RNA

RIN

>8

>8

>8

N/A

Stranded

yes

yes

yes

yes

Bacteria

no

yes

no

no

FFPE

no

no

no

yes

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