General Information

How long does it take from sending my samples to getting the result?

We can give you a return time, which depends on several factors such as the number of samples, the analysis used and the kind of data analysis you request. Please contact us for further information

How much do your services cost?

Based on your project details we will calculate the price and send you a quote. Please contact us to discuss your project and request a quote.

I cannot find my application of interest. Do you plan to offer it?

We are continually expanding our service portfolio. If you do not find your application of interest or do not know how to address your research question, our team is happy to help. Please contact us.

Sample Preparation and QC

Do you accept isolated samples or ready-to-use libraries?

You can send us samples for extraction. Make sure that the samples are taken, stored and shipped according to our instructions, which we will discuss with you.

You can also send us already isolated samples or ready-to-use libraries. Make sure that these samples are taken, processed, stored and shipped according to our instructions, which we will discuss with you.

In all cases you must discuss the methods you use with us first to ensure that they are compatible with the kind of analysis you want us to perform.

Please bear in mind that sample appropriate sample quantity and quality are crucial to success of your project. Check our sections to learn more about the required quantity and quality of nucleic acids for specific applications and about the measurements we perform with state-of-the-art equipment

How do you extract my samples?

Our lab team has extensive expertise in extracting and processing nucleic acids from diverse source materials: blood, normal and tumor tissue, amniotic cells, and excretions (saliva, urine, feces). Materials can be fresh, fresh-frozen, stored in stabilization reagents, such as PaxGene products, or fixed in FFPE.

We work with human, murine, and rat samples, but also with specimens from other known and unknown species, including plants and bacteria.

In certain kinds of samples degradation and contamination are typical problems. We are well versed in obtaining maximum yields and qualities from FFPE samples, ultra-low amounts of input material, and e.g. extracting human DNA from saliva samples containing a high amount of microflora/bacteria. Moreover, we have established adequate controls for potential sample degradation and contamination, which will be monitored in our QC process.

For simultaneous analyses of RNA and DNA from the same samples, we employ specialized extraction protocols.

Naturally, we choose the best suited kit for each kind of source material and desired application. Please contact us, if you want to learn more about the methods and kits we employ or consult our sections about transcriptomics and genomics. We are happy to explain the requirements that source materials have to meet for your application of choice. In addition, we offer advice on sample taking, storage, and shipping.

RNA in particular has to be stabilized quickly after taking samples. Special reagents will preserve RNA and DNA integrity and even protein and cell integrity, if desired, and special kits will permit simultaneous isolation. Such tools will allow you to analyze RNA, DNA, protein, and cellular characteristics in the same sample. This is very valuable when dealing with oncological samples, since small sizes of tissue samples can limit the amount of analyses you can perform on the same specimen. In addition, intra-tumor heterogeneity can limit the reliability of the data when DNA and RNA are isolated from different portions of the tumor. Co-isolation of DNA and RNA can overcome this problem.

What kind of library preparation do you offer?

We routinely prepare a diverse range of libraries, such as shotgun libraries for whole genomes, amplicon sequencing, enrichments for whole exome analyses, or gene panels and libraries for transcriptome, miRNA, and 16S rRNA analyses.
Of course, you can also send us your own ready-to-sequence libraries for analysis. Please check the appropriate sections for your application or contact us .

How do you check sample and library quantity and quality?

We have a quality control system tailored per application

For quality control we routinely perform rigorous tests to determine relevant parameters and base our decision on whether to use the provided material for our measurements. If our standards are not met, we will discuss this with you to find the optimal approach.

Check our sections to learn more about the required quantity and quality of nucleic acids for specific applications and about the measurements we perform with state-of-the-art equipment.

Why does DNA and RNA quality matter?

NGS and microarray analyses are highly specialized applications. DNA and RNA integrity and fragment size affect the results directly. Sample degradation should be avoided, but if no better input material is available, we can use methods to cope with less than optimal samples.

What are the right sample batch sizes?

At c.ATG we serve customers across a whole range of project sizes: from small, discovery-size, or pilot projects with one or a few samples to medium-sized or large, high-throughput, or validation-phase projects with hundreds of samples.

Certain applications require specific numbers of samples, i.e. batch sizes. These differ per application. Batch sizes depend on available kit sizes and hands-on time. Our prices can vary depending on the batch size of your experiment.

We will give you an indication of the average time at which you can expect the analysis to be completed. Please contact us for more information

Project Design and Bioinformatics

Do you help with project planning, bioinformatics and statistical analysis?

We offer help with project planning and strongly urge you to contact us during the planning phase of your project, especially to ensure that it is designed for proper statistical analysis. This will aid the interpretation and publication of the results.

For detailed information about data processing and analysis, please check our bioinformatics section.

Do you help with publishing the results of my project?

We offer help with publishing your results. Please contact us to discuss how we can assist you, for instance with preparing the Materials & Methods section or graphical representation of the analysis results.

Studying diseases

Do you also analyze diagnostic samples?

Requests for performing diagnostic analyses have to be directed to our partners: the Institute for Medical Genetics and Applied Genomics, Tübingen University Clinic, or to Genes & Therapy.

The Institute for Medical Genetics and Applied Genomics is the official partner for diagnostic tests covered by German health insurance.

Genes & Therapy serves patients without German Health insurance and patients who pay for the diagnostic tests themselves (so called “Selbstzahler”), if those tests are not yet covered by their German health insurance.

How do I use your services to study a specific disease?

We have ample experience with the study of neurological diseases, rare diseases and cancer in the context of of basic and clinical research projects.

Please consult our sections on whole exome sequencing (WES) and gene panel or RNA-seq analysis for more information.

For cancer studies we often use WES and transcriptome analyses together or perform gene panel analyses on a set of oncogenes and tumor suppressor genes.

Please contact us for more information.